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Home / Reconstitution calculator
Bench tool

Reconstitution calculator.

Working concentration, draw volume and aliquot count for a lyophilized vial. Laboratory preparation reference only — no dosing guidance is offered or implied.

5.0 units
5.00 mg/mL · draw 0.050 mL · 40 aliquots per vial

How the arithmetic works

Concentration in mg/mL is the vial mass divided by the diluent volume. A 10 mg vial reconstituted with 2 mL gives 5 mg/mL.

Draw volume is the target aliquot mass divided by that concentration. A 250 mcg aliquot from a 5 mg/mL solution is 0.25 mg ÷ 5 mg/mL = 0.05 mL, which reads as 5 units on a U-100 graduation.

Aliquot count is total vial mass divided by aliquot mass. The calculator flags any draw that exceeds the barrel capacity of the selected graduation.

Technique, diluent selection and storage of the prepared solution are covered in the reconstitution guide.